Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational designElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ob00376a

The 62nd residue of glycosyltransferase UGT BL 1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH t...

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Bibliographic Details
Main Authors Fan, Bo, Dong, Wenxin, Chen, Tianyi, Chu, Jianlin, He, Bingfang
Format Journal Article
LanguageEnglish
Published 04.04.2018
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Summary:The 62nd residue of glycosyltransferase UGT BL 1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product. Conduct structure-guided modification on the "hotspot" of glycosyltransferase UGT BL 1 to significantly adjust its regioselectivity toward polydatin production.
Bibliography:10.1039/c8ob00376a
Electronic supplementary information (ESI) available. See DOI
ISSN:1477-0520
1477-0539
DOI:10.1039/c8ob00376a