Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometryElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ob00329g

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies,...

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Bibliographic Details
Main Authors Hagen, Susan E, Liu, Kun, Jin, Yafei, Piersimoni, Lolita, Andrews, Philip C, Showalter, Hollis D
Format Journal Article
Published 26.09.2018
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Summary:Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins. Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/c8ob00329g
ISSN:1477-0520
1477-0539
DOI:10.1039/c8ob00329g