Unraveling the photoactive annihilation mechanism of nanostructures as effective green tools for inhibiting the proliferation of the phytopathogenic bacterium

• Published in: Nanoscale advances Vol. 1; no. 6; pp. 2258 - 2267
• Main Authors: Casas-Flores, Sergio, Domínguez-Espíndola, Ruth B, Camposeco-solis, Roberto, Patrón-Soberano, Olga A, Rodríguez-González, Vicente
• Format: Journal Article
• Published: 11.06.2019
• ISSN: 2516-0230
• DOI: 10.1039/c8na00307f

The infectious proliferation of phytopathogenic microorganisms depends on a complex sequence of biological events involving host defense, environmental conditions, and chemical and physical interactions between the surface of a plant and microorganisms, which in numerous cases display resistance to conventional microbicides. Among these microorganisms, Pseudomonas syringae ( P. syringae ) is a Gram-negative bacterium that attacks wounded parts of plants before invading healthy tissues. In order to control P. syringae , considering it to be a phytopathogenic model, an effective method featuring silver nanoparticles (AgNPs) functionalized on titanate nanotubes (Nts) used as photoactive antibacterial agents was investigated to understand the effective photoactive annihilation mechanism. The high dispersion of AgNPs over the Nts boosted charge carrier separation by generating reactive oxygen species (ROS) under visible-light, which stressed the bacteria and enhanced the biocidal effect by quickly preventing the rod-shaped P. syringae bacteria from proliferating. Biological transmission and scanning electron microscopy revealed damaged P. syringae cells that underwent the formation of outer membrane vesicles, caused by photo-assisted annihilation, which is considered to be an indication of a critical defense mechanism. The unusual synergistic properties of the Nts, and their low cost and practical synthesis, made these nanocomposites promising green tools that can positively and swiftly photokill P. syringae within 30 min. Effective annihilation in a short time acts in three simultaneous ways, photochemical, mechanical damage of sharp morphology and biocidal silver properties that made difficult the microbial resistance of Pseudomonas syringae .