A clickable glutamine (CliQ) derivative for the traceless reversible modification of peptides and proteinsElectronic supplementary information (ESI) available. See DOI: 10.1039/c8cc09404g

The Cu( i )-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures. However, irreversible protein modification limits the interpretation of results from subsequent biophysical and biochemical assays. We report a facile and modular chemical stra...

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Bibliographic Details
Main Authors Whedon, Samuel D, Parker, Marissa K, Tyson, Elizabeth L, Ritterhoff, Tobias, Shelton, Patrick M. M, Chatterjee, Champak
Format Journal Article
LanguageEnglish
Published 12.02.2019
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Summary:The Cu( i )-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures. However, irreversible protein modification limits the interpretation of results from subsequent biophysical and biochemical assays. We report a facile and modular chemical strategy to reversibly modify peptides and proteins with biotin and FLAG affinity tags at a clickable glutamine (CliQ) residue. The Cu( i )-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/c8cc09404g
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc09404g