Improving NanoCluster Beacon performance by blocking the unlabeled NC probesElectronic supplementary information (ESI) available: Explanation of the target specific signal (TSS), experimental methods, DNA sequences, supplemental figures. See DOI: 10.1039/c8cc08291j

While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold...

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Main Authors Chen, Yu-An, Vu, Huong T, Liu, Yen-Liang, Chen, Yuan-I, Nguyen, Trung Duc, Kuo, Yu-An, Hong, Soonwoo, Chen, Yin-An, Carnahan, Savannah, Petty, Jeffrey T, Yeh, Hsin-Chih
Format Journal Article
LanguageEnglish
Published 03.01.2019
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Summary:While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio. Adding a blocker strand significantly enhances the NanoCluster Beacon's detection signal.
Bibliography:Electronic supplementary information (ESI) available: Explanation of the target specific signal (TSS), experimental methods, DNA sequences, supplemental figures. See DOI
10.1039/c8cc08291j
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc08291j