Improving NanoCluster Beacon performance by blocking the unlabeled NC probesElectronic supplementary information (ESI) available: Explanation of the target specific signal (TSS), experimental methods, DNA sequences, supplemental figures. See DOI: 10.1039/c8cc08291j
While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold...
Saved in:
Main Authors | , , , , , , , , , , |
---|---|
Format | Journal Article |
Language | English |
Published |
03.01.2019
|
Online Access | Get full text |
Cover
Loading…
Summary: | While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio.
Adding a blocker strand significantly enhances the NanoCluster Beacon's detection signal. |
---|---|
Bibliography: | Electronic supplementary information (ESI) available: Explanation of the target specific signal (TSS), experimental methods, DNA sequences, supplemental figures. See DOI 10.1039/c8cc08291j |
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/c8cc08291j |