A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c8cc02034e

We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo -CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms. A fluor...

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Bibliographic Details
Main Authors Mehta, Radhika, Qureshi, Munaum H, Purchal, Meredith K, Greer, Sylvester M, Gong, Shanzhong, Ngo, Chinh, Que, Emily L
Format Journal Article
Published 24.05.2018
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Summary:We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo -CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms. A fluorescent probe for zinc-bound carbonic anhydrase is applied for tracking this protein in cell lysates and cells.
Bibliography:10.1039/c8cc02034e
Electronic supplementary information (ESI) available. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc02034e