Cellular traction forces: a useful parameter in cancer researchElectronic supplementary information (ESI) available. See DOI: 10.1039/c7nr06284b
The search for new cancer biomarkers is essential for fundamental research, diagnostics, as well as for patient treatment and monitoring. Whereas most cancer biomarkers are biomolecules, an increasing number of studies show that mechanical cues are promising biomarker candidates. Although cell defor...
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Main Authors | , , , , , , , , |
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Format | Journal Article |
Language | English |
Published |
14.12.2017
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Online Access | Get full text |
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Summary: | The search for new cancer biomarkers is essential for fundamental research, diagnostics, as well as for patient treatment and monitoring. Whereas most cancer biomarkers are biomolecules, an increasing number of studies show that mechanical cues are promising biomarker candidates. Although cell deformability has been shown to be a possible cancer biomarker, cellular forces as cancer biomarkers have been left largely unexplored. Here, we measure traction forces of cancer and normal-like cells at high spatial resolution using a robust method based on dense vertical arrays of nanowires. A force map is created using automated image analysis based on the localization of the fluorescent tips of the nanowires. We show that the force distribution and magnitude differ between MCF7 breast cancer cells and MCF10A normal-like breast epithelial cells, and that monitoring traction forces can be used to investigate the effects of anticancer drugs.
The effects of an anticancer drug is reflected in the traction forces of cancer and normal cells, which are measured using vertical nanowires. |
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Bibliography: | 10.1039/c7nr06284b Electronic supplementary information (ESI) available. See DOI |
ISSN: | 2040-3364 2040-3372 |
DOI: | 10.1039/c7nr06284b |