A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinasesElectronic supplementary information (ESI) available. See DOI: 10.1039/c7cc05504h

K27-linkage poly-ubiquitination plays important roles in DNA damage repair and autoimmunity. Identification of K27-linkage targeting deubiquitinases (DUBs) is essential for understanding their regulatory mechanisms. Here we report an aryl-azide-based photoaffinity diubiquitin (diUb) probe for profil...

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Bibliographic Details
Main Authors Tan, Xiao-Dan, Pan, Man, Gao, Shuai, Zheng, Yong, Shi, Jing, Li, Yi-Ming
Format Journal Article
LanguageEnglish
Published 12.09.2017
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Summary:K27-linkage poly-ubiquitination plays important roles in DNA damage repair and autoimmunity. Identification of K27-linkage targeting deubiquitinases (DUBs) is essential for understanding their regulatory mechanisms. Here we report an aryl-azide-based photoaffinity diubiquitin (diUb) probe for profiling K27-linkage targeting DUBs. This probe shows high selectivity and sensitivity towards K27-linkage DUBs in vitro . It can also be used in cell lysate for identifying and probing K27-linkage targeting DUBs in real proteomes. Our work suggests that the photoaffinity-based strategy may provide a new useful approach for the construction of other isopeptide linkage targeting DUB probes. We report a novel aryl-azide-based photoaffinity diubiquitin probe for profiling K27-linkage targeting DUBs in high selectivity and sensitivity.
Bibliography:10.1039/c7cc05504h
Electronic supplementary information (ESI) available. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc05504h