Pico-HPLC system integrating an equal inner diameter femtopipette into a 900 nm I.D. porous layer open tubular columnElectronic supplementary information (ESI) available. See DOI: 10.1039/c7cc00799j

A picoflow high performance liquid chromatography (pico-HPLC) system was developed, which could directly pipette femtoliter samples using a separation column tip driven by an electroosmotic pump. Amino acid enantiomers were separated in the 900 nm I.D. porous layer open tubular column at a flow rate...

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Bibliographic Details
Main Authors Li, Ruonan, Shao, Yunlong, Yu, Yanmin, Wang, Xiayan, Guo, Guangsheng
Format Journal Article
LanguageEnglish
Published 06.04.2017
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Summary:A picoflow high performance liquid chromatography (pico-HPLC) system was developed, which could directly pipette femtoliter samples using a separation column tip driven by an electroosmotic pump. Amino acid enantiomers were separated in the 900 nm I.D. porous layer open tubular column at a flow rate of 13.50 pL min −1 . A pico-HPLC method was developed using a bifunctional chromatographic column enabling femtoliter volume sampling and separation.
Bibliography:10.1039/c7cc00799j
Electronic supplementary information (ESI) available. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc00799j