Label-free biosensor based on dsDNA-templated copper nanoparticles for highly sensitive and selective detection of NAD+Electronic supplementary information (ESI) available: Additional figures. See DOI: 10.1039/c6ra17579a
We have developed a novel label-free fluorescence approach for nicotinamide adenine dinucleotide (NAD + ) detection by coupling poly(AT-TA) dsDNA-templated fluorescent copper nanoparticles (CuNPs) as a fluorescence indicator with a dumbbell DNA probe designed for ligation. Herein, we designed a dumb...
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Main Authors | , , , , , |
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Format | Journal Article |
Published |
23.09.2016
|
Online Access | Get full text |
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Summary: | We have developed a novel label-free fluorescence approach for nicotinamide adenine dinucleotide (NAD
+
) detection by coupling poly(AT-TA) dsDNA-templated fluorescent copper nanoparticles (CuNPs) as a fluorescence indicator with a dumbbell DNA probe designed for ligation. Herein, we designed a dumbbell probe which can form a self-complementary structure at both ends. In the absence of NAD
+
, the extension reaction can be performed from the exposed 3′-end, and instantly opened the hybridized structure at the 5′-end, producing a chimeric poly(AT-TA) double-stranded fragment which can be used as a highly-efficient template for the formation of CuNPs, showing high fluorescence. Whereas in the presence of NAD
+
, together with the
E. coli
DNA ligase, the 5′-phosphoryl end and the 3′-end of the DNA probe can be ligated to block the extension reaction, showing slight fluorescence. This novel NAD
+
assay is label-free, fast and easy to operate, highly sensitive and selective, which holds wide applicability as a robust analytical tool in NAD
+
related biological processes and clinical diagnosis.
A novel label-free biosensor for high sensing of NAD
+
based on dsDNA-templated CuNPs and DNA ligation reaction. |
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Bibliography: | Electronic supplementary information (ESI) available: Additional figures. See DOI 10.1039/c6ra17579a |
ISSN: | 2046-2069 |
DOI: | 10.1039/c6ra17579a |