Highly dynamic metal exchange in anthrax lethal factor involves the occupation of an inhibitory metal binding siteElectronic supplementary information (ESI) available: General experimental details, and supplementary figures and tables. See DOI: 10.1039/c6cc05460a
Metal exchange is a common strategy to replace the zinc ion of many zinc proteins with other transition metals amenable to spectroscopic investigations. We here demonstrate that in anthrax lethal factor (and likely other zinc proteases), metal exchange is a fast process, and involves the occupation...
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| Main Authors | , |
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| Format | Journal Article |
| Published |
27.09.2016
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| Online Access | Get full text |
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| Summary: | Metal exchange is a common strategy to replace the zinc ion of many zinc proteins with other transition metals amenable to spectroscopic investigations. We here demonstrate that in anthrax lethal factor (and likely other zinc proteases), metal exchange is a fast process, and involves the occupation of an inhibitory metal site by the incoming ion prior to the release of zinc.
Rapid metal exchange in anthrax lethal factor and potentially other zinc enzymes proceeds
via
the formation of transient bimetallic species. |
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| Bibliography: | 10.1039/c6cc05460a Electronic supplementary information (ESI) available: General experimental details, and supplementary figures and tables. See DOI |
| ISSN: | 1359-7345 1364-548X |
| DOI: | 10.1039/c6cc05460a |