Highly solvatochromic and tunable fluorophores based on a 4,5-quinolimide scaffold: novel CDK5 probesThis manuscript is dedicated to Professor M. Teresa García-López on occasion of her retirement after a distinguished scientific career on Medicinal Chemistry.Electronic supplementary information (ESI) available: Experimental procedures, photophysical and calculation data, figures of titration and cell imaging of a quinolimide labelled CDK5 peptide, as well as, NMR spectra. See DOI: 10.1039/c6cc04

Novel 4,5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introductio...

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Main Authors González-Vera, Juan A, Fueyo-González, Francisco, Alkorta, Ibon, Peyressatre, Marion, Morris, May C, Herranz, Rosario
Format Journal Article
Published 26.07.2016
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Summary:Novel 4,5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introduction of the quinolimide-labelled peptide into living glioblastoma cells probed the interaction with endogenous p25. Novel 4,5-quinolimide-based solvatochromic fluorophores conjugated to a CDK5 derived peptide demonstrated sensitivity for monitoring CDK5/p25 interaction in living cells.
Bibliography:10.1039/c6cc04566a
Electronic supplementary information (ESI) available: Experimental procedures, photophysical and calculation data, figures of titration and cell imaging of a quinolimide labelled CDK5 peptide, as well as, NMR spectra. See DOI
This manuscript is dedicated to Professor M. Teresa García-López on occasion of her retirement after a distinguished scientific career on Medicinal Chemistry.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc04566a