Determination of phenolic compounds in extracts of Amazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometryElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ay02937j
A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were...
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Main Authors | , , , , |
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Format | Journal Article |
Language | English |
Published |
16.02.2017
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Online Access | Get full text |
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Summary: | A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (
Mansoa alliacea
,
Bauhinia variegata
var.
variegata
,
Bauhinia variegata
var.
alboflava
,
Connarus perrottetii
var.
angustifolius
,
Cecropia obtusa
and
Cecropia palmata
). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min
−1
. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L
−1
. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (
r
> 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid,
p
-coumaric acid and
trans
-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of
Bauhinia variegata
(up to 2.52 mg g
−1
of plant), chlorogenic acid in species of genus
Cecropia
(up to 0.57 mg g
−1
of plant) and catechin in
Connarus perrottetii
var.
angustifolius
(1.77 mg g
−1
of plant).
A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. |
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Bibliography: | 10.1039/c6ay02937j Electronic supplementary information (ESI) available. See DOI |
ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/c6ay02937j |