Chemical profiling of cerebrospinal fluid by multiple reaction monitoring mass spectrometryData and biospecimen used in the preparation of this article were obtained from the Fox Investigation for New Discovery of Biomarkers databases. For up-to-date information on the study, visit www.michaeljfox.org/biofind.Electronic supplementary information (ESI) available. See DOI: 10.1039/c6an01618a

• Main Authors: Ferreira, Christina R, Yannell, Karen E, Mollenhauer, Brit, Espy, Ryan D, Cordeiro, Fernanda B, Ouyang, Z, Cooks, R. G
• Format: Journal Article
• Published: 30.08.2016
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c6an01618a

We report an accelerated biomarker discovery workflow and results of sample screening by mass spectrometry based on multiple reaction monitoring (MRM). This methodology shows promising initial results for the currently unsolved challenge of Parkinson's disease (PD) laboratory diagnosis by biomarker screening. Small molecules present in cerebrospinal fluid (CSF) at low parts per million levels are monitored using specific transitions connecting ion pairs. A set of such transitions constitutes a multidimensional chemical profile used to distinguish and characterize different CSF samples using multivariate statistical methods. We report an accelerated biomarker discovery workflow and results of sample screening by mass spectrometry based on multiple reaction monitoring (MRM).