Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule levelElectronic supplementary information (ESI) available. See DOI: 10.1039/c5sc02641e
Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRN...
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Format | Journal Article |
Language | English |
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13.10.2015
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Abstract | Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRNA at the single-molecule level. In this strategy, an isothermal exponential amplification reaction (EXPAR) is rationally designed towards specific miRNAs and all products of the EXPAR are integrated onto a single microbead for signal amplification and fluorescence enrichment. This pushes the detection of miRNAs down to 1 aM in a 5 μL sample, corresponding to 3 copies of the miRNA molecule. This new strategy also affords high selectivity and it is capable of distinguishing among homologous miRNA family members even with a single-base difference. Due to its ultrahigh sensitivity and selectivity, the proposed SMBS platform has been successfully applied to the detection of miRNA extracted from a single cell.
A single microbead-based sensing platform has been developed, which enables the detection of microRNA at the single-molecule level. |
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AbstractList | Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRNA at the single-molecule level. In this strategy, an isothermal exponential amplification reaction (EXPAR) is rationally designed towards specific miRNAs and all products of the EXPAR are integrated onto a single microbead for signal amplification and fluorescence enrichment. This pushes the detection of miRNAs down to 1 aM in a 5 μL sample, corresponding to 3 copies of the miRNA molecule. This new strategy also affords high selectivity and it is capable of distinguishing among homologous miRNA family members even with a single-base difference. Due to its ultrahigh sensitivity and selectivity, the proposed SMBS platform has been successfully applied to the detection of miRNA extracted from a single cell.
A single microbead-based sensing platform has been developed, which enables the detection of microRNA at the single-molecule level. |
Author | Li, Zhengping Sun, Lingbo Duan, Xinrui Zhang, Xiaobo Liu, Chenghui |
AuthorAffiliation | Ministry of Education Shaanxi Normal University School of Chemistry and Chemical Engineering Key Laboratory of Applied Surface and Colloid Chemistry Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province |
AuthorAffiliation_xml | – name: School of Chemistry and Chemical Engineering – name: Ministry of Education – name: Key Laboratory of Applied Surface and Colloid Chemistry – name: Shaanxi Normal University – name: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province |
Author_xml | – sequence: 1 givenname: Xiaobo surname: Zhang fullname: Zhang, Xiaobo – sequence: 2 givenname: Chenghui surname: Liu fullname: Liu, Chenghui – sequence: 3 givenname: Lingbo surname: Sun fullname: Sun, Lingbo – sequence: 4 givenname: Xinrui surname: Duan fullname: Duan, Xinrui – sequence: 5 givenname: Zhengping surname: Li fullname: Li, Zhengping |
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