Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule levelElectronic supplementary information (ESI) available. See DOI: 10.1039/c5sc02641e

Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRN...

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Bibliographic Details
Main Authors Zhang, Xiaobo, Liu, Chenghui, Sun, Lingbo, Duan, Xinrui, Li, Zhengping
Format Journal Article
LanguageEnglish
Published 13.10.2015
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Summary:Detection of a single nucleic acid molecule is of great significance for both fundamental biochemistry studies and clinical diagnostics. By using microRNA (miRNA) as a model target, herein, we have developed a single-microbead-based sensing (SMBS) platform, which simply enables the detection of miRNA at the single-molecule level. In this strategy, an isothermal exponential amplification reaction (EXPAR) is rationally designed towards specific miRNAs and all products of the EXPAR are integrated onto a single microbead for signal amplification and fluorescence enrichment. This pushes the detection of miRNAs down to 1 aM in a 5 μL sample, corresponding to 3 copies of the miRNA molecule. This new strategy also affords high selectivity and it is capable of distinguishing among homologous miRNA family members even with a single-base difference. Due to its ultrahigh sensitivity and selectivity, the proposed SMBS platform has been successfully applied to the detection of miRNA extracted from a single cell. A single microbead-based sensing platform has been developed, which enables the detection of microRNA at the single-molecule level.
Bibliography:10.1039/c5sc02641e
Electronic supplementary information (ESI) available. See DOI
ISSN:2041-6520
2041-6539
DOI:10.1039/c5sc02641e