Synthetic tools for studying the chemical biology of InsP8Electronic supplementary information (ESI) available: Data deposition: atomic coordinates and structure factors have been deposited in the Protein Data Bank, www.pdb.org (PDB ID codes 5BYA and 5BYB). See DOI: 10.1039/c5cc05017k

To synthesise stabilised mimics of InsP 8 , the most phosphorylated inositol phosphate signalling molecule in Nature, we replaced its two diphosphate (PP) groups with either phosphonoacetate (PA) or methylenebisphosphonate (PCP) groups. Utility of the PA and PCP analogues was verified by structural...

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Bibliographic Details
Main Authors Riley, Andrew M, Wang, Huanchen, Shears, Stephen B, Potter, Barry V
Format Journal Article
LanguageEnglish
Published 23.07.2015
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Summary:To synthesise stabilised mimics of InsP 8 , the most phosphorylated inositol phosphate signalling molecule in Nature, we replaced its two diphosphate (PP) groups with either phosphonoacetate (PA) or methylenebisphosphonate (PCP) groups. Utility of the PA and PCP analogues was verified by structural and biochemical analyses of their interactions with enzymes of InsP 8 metabolism. Synthetic mimics of InsP 8 , Nature's most phosphorylated inositol phosphate, interact differentially with the kinase PPIP5K2 and provide stabilised, complementary tools to investigate this orphan signal.
Bibliography:Electronic supplementary information (ESI) available: Data deposition: atomic coordinates and structure factors have been deposited in the Protein Data Bank
and
10.1039/c5cc05017k
PDB ID codes
See DOI
5BYA
www.pdb.org
5BYB
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc05017k