The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafishElectronic supplementary information (ESI) available: Characterization of the new compound, additional graphs and detailed experimental procedures. See DOI: 10.1039/c4cc08075k

We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate rea...

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Bibliographic Details
Main Authors Sander, Veronika, Patke, Shantanu, Sahu, Srikanta, Teoh, Chai Lean, Peng, Zhenzhen, Chang, Young-Tae, Davidson, Alan J
Format Journal Article
LanguageEnglish
Published 05.12.2014
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Summary:We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function. The fluorescent compound PT-Yellow is a novel tool to label the proximal tubule cells of the zebrafish kidney in vivo .
Bibliography:10.1039/c4cc08075k
Electronic supplementary information (ESI) available: Characterization of the new compound, additional graphs and detailed experimental procedures. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08075k