Direct conversion of Cytochrome c spectral shifts to fluorescence using photochromic FRETElectronic supplementary information (ESI) available: Sample preparation, fluorescence lifetime, data analysis, supplementary Fig. S1-S7 and Tables S1 and S2. See DOI: 10.1039/c4cc06146b

Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c . Photochromic fluorescenc...

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Bibliographic Details
Main Authors Manioglu, Selen, Atis, Muge, Aas, Mehdi, Kiraz, Alper, Bayraktar, Halil
Format Journal Article
LanguageEnglish
Published 18.09.2014
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Summary:Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c . Photochromic fluorescence resonance energy transfer was used to determine the oxidation state change of Cytochrome c that modulated the Venus emission.
Bibliography:Electronic supplementary information (ESI) available: Sample preparation, fluorescence lifetime, data analysis, supplementary Fig. S1-S7 and Tables S1 and S2. See DOI
10.1039/c4cc06146b
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc06146b