Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA displayElectronic supplementary information (ESI) available: Library construction, in vitro selection, binding assay, circular dichroism (CD) and peptide structure prediction. See DOI: 10.1039/c4cc00799a

Peptide aptamers that specifically bind to the amino group on a solid-phase were screened by in vitro selection using the cDNA display method. The identified peptides have a unique structure containing two cyclic loops with disulphide bonds and a linkage region, which were indispensable for molecula...

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Main Authors Mochizuki, Yuki, Nishigaki, Koichi, Nemoto, Naoto
Format Journal Article
LanguageEnglish
Published 29.04.2014
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Abstract Peptide aptamers that specifically bind to the amino group on a solid-phase were screened by in vitro selection using the cDNA display method. The identified peptides have a unique structure containing two cyclic loops with disulphide bonds and a linkage region, which were indispensable for molecular recognition. Double disulphide-bridged loops of peptide aptamer are indispensable for the amino group recognition.
AbstractList Peptide aptamers that specifically bind to the amino group on a solid-phase were screened by in vitro selection using the cDNA display method. The identified peptides have a unique structure containing two cyclic loops with disulphide bonds and a linkage region, which were indispensable for molecular recognition. Double disulphide-bridged loops of peptide aptamer are indispensable for the amino group recognition.
Author Nishigaki, Koichi
Nemoto, Naoto
Mochizuki, Yuki
AuthorAffiliation Graduate School of Science and Engineering
Saitama University
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  surname: Nishigaki
  fullname: Nishigaki, Koichi
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  givenname: Naoto
  surname: Nemoto
  fullname: Nemoto, Naoto
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Notes 10.1039/c4cc00799a
selection, binding assay, circular dichroism (CD) and peptide structure prediction. See DOI
Electronic supplementary information (ESI) available: Library construction
in vitro
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Snippet Peptide aptamers that specifically bind to the amino group on a solid-phase were screened by in vitro selection using the cDNA display method. The identified...
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Title Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA displayElectronic supplementary information (ESI) available: Library construction, in vitro selection, binding assay, circular dichroism (CD) and peptide structure prediction. See DOI: 10.1039/c4cc00799a
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