A novel P450-based biocatalyst for the selective production of chiral 2-alkanolsElectronic supplementary information (ESI) available: Materials and methods. GC/MS chromatograms, time dependence of octane conversion, and supplementation experiments. See DOI: 10.1039/c4cc00647j
A P450 monooxygenase from Nocardia farcinica (CYP154A8) catalyses the stereo- and regioselective hydroxylation of n -alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C 7 -C 9 alkanes was over 90%. The enzyme showed st...
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| Main Authors | , |
|---|---|
| Format | Journal Article |
| Language | English |
| Published |
20.03.2014
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| Online Access | Get full text |
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| Abstract | A P450 monooxygenase from
Nocardia farcinica
(CYP154A8) catalyses the stereo- and regioselective hydroxylation of
n
-alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C
7
-C
9
alkanes was over 90%. The enzyme showed strict
S
-selectivity for all tested substrates, with enantiomeric excess (ee) of up to 91%.
CYP154A8 catalyses regio- and stereoselective hydroxylation of
n
-alkanes (C
7
-C
10
) to the corresponding 2-(
S
)-alkanols (up to 91% ee) with high TTN. |
|---|---|
| AbstractList | A P450 monooxygenase from
Nocardia farcinica
(CYP154A8) catalyses the stereo- and regioselective hydroxylation of
n
-alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C
7
-C
9
alkanes was over 90%. The enzyme showed strict
S
-selectivity for all tested substrates, with enantiomeric excess (ee) of up to 91%.
CYP154A8 catalyses regio- and stereoselective hydroxylation of
n
-alkanes (C
7
-C
10
) to the corresponding 2-(
S
)-alkanols (up to 91% ee) with high TTN. |
| Author | von Bühler, Clemens J Urlacher, Vlada B |
| AuthorAffiliation | Heinrich-Heine University Düsseldorf Institute of Biochemistry |
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| Author_xml | – sequence: 1 givenname: Clemens J surname: von Bühler fullname: von Bühler, Clemens J – sequence: 2 givenname: Vlada B surname: Urlacher fullname: Urlacher, Vlada B |
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| DOI | 10.1039/c4cc00647j |
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| Notes | 10.1039/c4cc00647j Electronic supplementary information (ESI) available: Materials and methods. GC/MS chromatograms, time dependence of octane conversion, and supplementation experiments. See DOI |
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| Snippet | A P450 monooxygenase from
Nocardia farcinica
(CYP154A8) catalyses the stereo- and regioselective hydroxylation of
n
-alkanes, still a challenging task in... |
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| Title | A novel P450-based biocatalyst for the selective production of chiral 2-alkanolsElectronic supplementary information (ESI) available: Materials and methods. GC/MS chromatograms, time dependence of octane conversion, and supplementation experiments. See DOI: 10.1039/c4cc00647j |
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