A novel P450-based biocatalyst for the selective production of chiral 2-alkanolsElectronic supplementary information (ESI) available: Materials and methods. GC/MS chromatograms, time dependence of octane conversion, and supplementation experiments. See DOI: 10.1039/c4cc00647j

A P450 monooxygenase from Nocardia farcinica (CYP154A8) catalyses the stereo- and regioselective hydroxylation of n -alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C 7 -C 9 alkanes was over 90%. The enzyme showed st...

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Bibliographic Details
Main Authors von Bühler, Clemens J, Urlacher, Vlada B
Format Journal Article
LanguageEnglish
Published 20.03.2014
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Summary:A P450 monooxygenase from Nocardia farcinica (CYP154A8) catalyses the stereo- and regioselective hydroxylation of n -alkanes, still a challenging task in chemical catalysis. In a biphasic reaction system, the regioselectivity for the C2-position of C 7 -C 9 alkanes was over 90%. The enzyme showed strict S -selectivity for all tested substrates, with enantiomeric excess (ee) of up to 91%. CYP154A8 catalyses regio- and stereoselective hydroxylation of n -alkanes (C 7 -C 10 ) to the corresponding 2-( S )-alkanols (up to 91% ee) with high TTN.
Bibliography:10.1039/c4cc00647j
Electronic supplementary information (ESI) available: Materials and methods. GC/MS chromatograms, time dependence of octane conversion, and supplementation experiments. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc00647j