Total synthesis and characterization of thielocin B1 as a protein-protein interaction inhibitor of PAC3 homodimerElectronic supplementary information (ESI) available: Synthetic protocols and characterization data, methods for docking study and molecular dynamics simulation, protein expression and purification, and NMR titration, and 1H and 13C NMR spectra of 1, 2b, 3, 4, and 6-19. See DOI: 10.1039/c3sc53237b
We have characterized the inhibition of the protein-protein interaction of the homodimer of proteasome assembling chaperone (PAC) 3 with thielocin B1, discovered from natural product sources. Molecular modeling using docking studies and molecular dynamics simulation suggested that thielocin B1 exhib...
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Main Authors | , , , , , , , , , |
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Format | Journal Article |
Language | English |
Published |
01.04.2014
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Online Access | Get full text |
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Summary: | We have characterized the inhibition of the protein-protein interaction of the homodimer of proteasome assembling chaperone (PAC) 3 with thielocin B1, discovered from natural product sources. Molecular modeling using docking studies and molecular dynamics simulation suggested that thielocin B1 exhibits distinct binding positions on the interface of the homodimer and the complex can be stabilized by five interactive residues on PAC3. Thielocin B1 was synthesized for the first time and was utilized for nuclear magnetic resonance (NMR) titration of the PAC3 homodimer. The data revealed significant chemical shift perturbations observed in eight residues on PAC3. We also synthesized a spin-labeled derivative to observe paramagnetic relaxation enhancement (PRE) effects. As a result, distinct decrease of the intensities of the NH peaks was observed in sixteen residues of PAC3 in the presence of the spin-labeled derivative. Both the NMR experiments and further
in silico
docking studies have suggested that thielocin B1 approaches one face of the PAC3 homodimer, not the monomer, releasing the subunit of the interface of the PAC3 homodimer by a rare pre-dissociation-independent mechanism.
We have characterized the inhibition of the protein-protein interaction of the homodimer of proteasome assembling chaperone (PAC) 3 with thielocin B1. |
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Bibliography: | 10.1039/c3sc53237b 13 6-19 , See DOI 2b 1 3 4 and H and Electronic supplementary information (ESI) available: Synthetic protocols and characterization data, methods for docking study and molecular dynamics simulation, protein expression and purification, and NMR titration, and C NMR spectra of |
ISSN: | 2041-6520 2041-6539 |
DOI: | 10.1039/c3sc53237b |