Scalable synthesis of γ-thiolysine starting from lysine and a side by side comparison with δ-thiolysine in non-enzymatic ubiquitinationElectronic supplementary information (ESI) available: Experimental procedures and characterization for all compounds, SDS PAGE analysis, HPLC-MS analysis, CD spectra, NMR spectra. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c3sc51599k

We developed a scalable synthesis of γ-thiolysine starting straight from lysine. The application of γ-thiolysine was compared to δ-thiolysine in the chemical synthesis of K48 and K33 linked diubiquitin conjugates. Both γ- and δ-thiolysine were found to perform equally efficiently as handles for non-...

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Main Authors Merkx, Remco, de Bruin, Gerjan, Kruithof, Art, van den Bergh, Toine, Snip, Erwin, Lutz, Martin, El Oualid, Farid, Ovaa, Huib
Format Journal Article
LanguageEnglish
Published 28.10.2013
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Summary:We developed a scalable synthesis of γ-thiolysine starting straight from lysine. The application of γ-thiolysine was compared to δ-thiolysine in the chemical synthesis of K48 and K33 linked diubiquitin conjugates. Both γ- and δ-thiolysine were found to perform equally efficiently as handles for non-enzymatic ubiquitination. A scalable synthesis of γ-thiolysine and a side by side comparison to δ-thiolysine as a handle for non-enzymatic ubiquitination is reported.
Bibliography:10.1039/c3sc51599k
Electronic supplementary information (ESI) available: Experimental procedures and characterization for all compounds, SDS PAGE analysis, HPLC-MS analysis, CD spectra, NMR spectra. For ESI and crystallographic data in CIF or other electronic format see DOI
ISSN:2041-6520
2041-6539
DOI:10.1039/c3sc51599k