Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminusElectronic supplementary information (ESI) available: Synthetic procedure for newly synthesized compounds and their spectroscopic data. See DOI: 10.1039/c3ob41901k

To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one o...

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Main Authors Iijima, Yoshihiro, Kojima, Shun, Kodama, Erika, Kurohagi, Sayako, Kanamori, Takashi, Masaki, Yoshiaki, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji
Format Journal Article
LanguageEnglish
Published 13.11.2013
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Summary:To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one of these modified oligodeoxynucleotides as a reverse transcription primer enabled a novel RT-PCR that can discriminate among miRNA length variants. Modified oligonucleotides containing a cyclohexyl phosphate were applied to a new RT-PCR that allows discrimination of miRNA length variants.
Bibliography:10.1039/c3ob41901k
Electronic supplementary information (ESI) available: Synthetic procedure for newly synthesized compounds and their spectroscopic data. See DOI
ISSN:1477-0520
1477-0539
DOI:10.1039/c3ob41901k