Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solventElectronic supplementary information (ESI) available. See DOI: 10.1039/c2gc35559k

Subtilisin Carlsberg (SC) was immobilized and stabilized on magnetically-separable mesoporous silica (Mag-MSU-F) in the form of nanoscale enzyme reactors (NERs) based on the ship-in-a-bottle mechanism. Stabilized NERs of SC (NER-SC) were freeze-dried and successfully used for the transesterification...

Full description

Saved in:
Bibliographic Details
Main Authors Hwang, Ee Taek, Lee, Byoungsoo, Zhang, Meiling, Jun, Seung-Hyun, Shim, Jongmin, Lee, Jinwoo, Kim, Jungbae, Gu, Man Bock
Format Journal Article
LanguageEnglish
Published 01.07.2012
Online AccessGet full text

Cover

More Information
Summary:Subtilisin Carlsberg (SC) was immobilized and stabilized on magnetically-separable mesoporous silica (Mag-MSU-F) in the form of nanoscale enzyme reactors (NERs) based on the ship-in-a-bottle mechanism. Stabilized NERs of SC (NER-SC) were freeze-dried and successfully used for the transesterification of N -acetyl- l -phenylalanine ethyl ester with n -propanol in isooctane. Magnetic separation of Mag-MSU-F facilitated the repeated usages of stable NER-SC. This is the first demonstration for the use of stable and magnetically-separable NERs in an organic solvent, which has the potential for environmentally-friendly synthesis using enzymes in organic solvents. Highly-stable and magnetically-separable nanoscale enzyme reactors for enzyme-catalyzed synthetic reactions.
Bibliography:10.1039/c2gc35559k
Electronic supplementary information (ESI) available. See DOI
ISSN:1463-9262
1463-9270
DOI:10.1039/c2gc35559k