Quantum dot-DNA origami binding: a single particle, 3D, real-time tracking studyElectronic supplementary information (ESI) available: Experimental details including DNA sequences, representative data set of binding vs switching, agarose gel analysis, SEM images, and typical fluorescence intensity measurement data. See DOI: 10.1039/c2cc37517f

The binding process of quantum dots and DNA origami was monitored using a 3D, real-time, single-particle tracking system. Single-molecule binding events were directly observed and precise measurements of the diffusion coefficient and second-order photon correlation function, g 2 ( τ ), were combined...

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Bibliographic Details
Main Authors Du, Kan, Ko, Seung Hyeon, Gallatin, Gregg M, Yoon, Heayoung P, Alexander Liddle, J, Berglund, Andrew J
Format Journal Article
LanguageEnglish
Published 02.01.2013
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Summary:The binding process of quantum dots and DNA origami was monitored using a 3D, real-time, single-particle tracking system. Single-molecule binding events were directly observed and precise measurements of the diffusion coefficient and second-order photon correlation function, g 2 ( τ ), were combined to distinguish free quantum dots from different conjugates of n Qdot-origami. The binding process of quantum dots and DNA origami, including single-molecule events, was monitored using a 3D, real-time, single-particle tracking system.
Bibliography:switching, agarose gel analysis, SEM images, and typical fluorescence intensity measurement data. See DOI
Electronic supplementary information (ESI) available: Experimental details including DNA sequences, representative data set of binding
10.1039/c2cc37517f
vs
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc37517f