Using a nanopore for single molecule detection and single cell transfectionThis article is part of a themed issue highlighting the targeted study of single units, such as molecules, cells, organelles and pores - The "Single" Issue, guest edited by Henry White

We assert that it is possible to trap and identify proteins, and even (conceivably) manipulate proteins secreted from a single cell ( i.e. the secretome) through transfection via electroporation by exploiting the exquisite control over the electrostatic potential available in a nanopore. These capab...

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Bibliographic Details
Main Authors Nelson, Edward M, Kurz, Volker, Shim, Jiwook, Timp, Winston, Timp, Gregory
Format Journal Article
LanguageEnglish
Published 06.06.2012
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Summary:We assert that it is possible to trap and identify proteins, and even (conceivably) manipulate proteins secreted from a single cell ( i.e. the secretome) through transfection via electroporation by exploiting the exquisite control over the electrostatic potential available in a nanopore. These capabilities may be leveraged for single cell analysis and transfection with single molecule resolution, ultimately enabling a careful scrutiny of tissue heterogeneity. A synthetic nanopore can be used to both transfect a single cell and detect secreted analytes.
Bibliography:This article is part of a themed issue highlighting the targeted study of single units, such as molecules, cells, organelles and pores - The "Single" Issue, guest edited by Henry White.
ISSN:0003-2654
1364-5528
DOI:10.1039/c2an35571j