Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporterThis article is part of the ChemComm Emerging Investigators 2012 themed issue.Electronic supplementary information (ESI) available: Supplementary figures and experimental methods. See DOI: 10.1039/c1cc14939c

Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para -ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient...

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Bibliographic Details
Main Authors Grammel, Markus, Dossa, Paul D, Taylor-Salmon, Emma, Hang, Howard C
Format Journal Article
LanguageEnglish
Published 11.01.2012
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Summary:Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para -ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection. The orthogonal amino acid reporter para -ethynylphenylalanine (PEP) allows cell-selective metabolic proteome labeling in heterogeneous cell populations.
Bibliography:Electronic supplementary information (ESI) available: Supplementary figures and experimental methods. See DOI
10.1039/c1cc14939c
This article is part of the
ChemComm
Emerging Investigators 2012 themed issue.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc14939c