Analysis and hit filtering of a very large library of compounds screened against Mycobacterium tuberculosisElectronic supplementary information (ESI) available: Supplementary figures and methods. The Bayesian models created in Discovery Studio are available from the authors upon written request. See DOI: 10.1039/c0mb00104jContributions of co-authors: SE directed and performed the computational analyses and wrote all versions of the manuscript, TK assisted with medicinal chemistry expertise,data
There is an urgent need for new drugs against tuberculosis which annually claims 1.7-1.8 million lives. One approach to identify potential leads is to screen in vitro small molecules against Mycobacterium tuberculosis (Mtb). Until recently there was no central repository to collect information on co...
Saved in:
Main Authors | , , , , , , , , , , , , |
---|---|
Format | Journal Article |
Language | English |
Published |
01.11.2010
|
Online Access | Get full text |
Cover
Loading…
Summary: | There is an urgent need for new drugs against tuberculosis which annually claims 1.7-1.8 million lives. One approach to identify potential leads is to screen
in vitro
small molecules against
Mycobacterium tuberculosis
(Mtb). Until recently there was no central repository to collect information on compounds screened. Consequently, it has been difficult to analyze molecular properties of compounds that inhibit the growth of Mtb
in vitro
. We have collected data from publically available sources on over 300 000 small molecules deposited in the Collaborative Drug Discovery TB Database. A cheminformatics analysis on these compounds indicates that inhibitors of the growth of Mtb have statistically higher mean logP, rule of 5 alerts, while also having lower HBD count, atom count and lower PSA (ChemAxon descriptors), compared to compounds that are classed as inactive. Additionally, Bayesian models for selecting Mtb active compounds were evaluated with over 100 000 compounds and, they demonstrated 10 fold enrichment over random for the top ranked 600 compounds. This represents a promising approach for finding compounds active against Mtb in whole cells screened under the same
in vitro
conditions. Various sets of Mtb hit molecules were also examined by various filtering rules used widely in the pharmaceutical industry to identify compounds with potentially reactive moieties. We found differences between the number of compounds flagged by these rules in Mtb datasets, malaria hits, FDA approved drugs and antibiotics. Combining these approaches may enable selection of compounds with increased probability of inhibition of whole cell Mtb activity.
This work describes various filters that were used to evaluate Tuberculosis screening datasets, malaria hits, FDA approved drugs and antibiotics. |
---|---|
Bibliography: | Electronic supplementary information (ESI) available: Supplementary figures and methods. The Bayesian models created in Discovery Studio are available from the authors upon written request. See DOI Contributions of co-authors: SE directed and performed the computational analyses and wrote all versions of the manuscript, TK assisted with medicinal chemistry expertise,data interpretation, prepared some of the structure datasets for Smartsfilter analysis and provided extensive edits for all versions of the manuscript, CAL provided information and expertise for compound filtering and performed the initial analysis for several Mtb lead compounds which inspired their use here as well as comments on data analysis, JB assisted with large dataset analysis, AS and KG provided dataset sourcing, validation and upload, DB, KD and MH developed the CDD TB database and provided the mock-up for Alerts as well as text on development of the software. JY developed and provided the Smartsfilter and enabled use in this project, NG curated the TB patent database and assisted with curation of other TB literature databases, BAB provided early edits to the manuscript and obtained funding for this work. 10.1039/c0mb00104j |
ISSN: | 1742-206X 1742-2051 |
DOI: | 10.1039/c0mb00104j |