The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flowThis paper is part of an Organic & Biomolecular Chemistry web theme issue on enabling technologies for organic synthesis

• Main Authors: Ngamsom, B, Hickey, A. M, Greenway, G. M, Littlechild, J. A, McCreedy, T, Watts, P, Wiles, C
• Format: Journal Article
• Language: English
• Published: 06.05.2010
• ISSN: 1477-0520; 1477-0539
• DOI: 10.1039/b924100k

Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of ( rac )-2-phenylpropionaldehyde to ( S )-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds. Employing a novel conducting controlled pore glass-poly(pyrrole) material for the co-immobilisation of HLADH and NAD(H), we were able to fabricate a reagent-less flow reactor capable of the continuous biosynthesis of chiral compounds under an applied voltage.