Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germ line cells

Myh6 -Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26 fstdTom reporter ( tdTom ) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results...

Full description

Saved in:
Bibliographic Details
Published inJournal of molecular and cellular cardiology Vol. 175; pp. 62 - 66
Main Authors Sheldon, Caroline, Kessinger, Chase W., Sun, Yan, Kontaridis, Maria I., Ma, Qianyi, Hammoud, Saher Sue, Gao, Zibei, Zhang, Hui, Lin, Zhiqiang
Format Journal Article
LanguageEnglish
Published 28.12.2022
Online AccessGet full text

Cover

More Information
Summary:Myh6 -Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26 fstdTom reporter ( tdTom ) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germ line cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known “heart-specific” Myh6 promoter drives Cre expression in the testis.
ISSN:0022-2828
1095-8584
DOI:10.1016/j.yjmcc.2022.12.005