The role of fat on cardiomyopathy outcome in mice models of chronic Trypanosoma cruzi infection
The underlying pathogenic mechanisms of cardiomyopathy in Chagas Disease are still unsolved. In order to better clarify the role of fat on the evolution of cardiomyopathy, the present study employed three murine models of chronic Trypanosoma cruzi infection: (1) aP2-RIDα/β transgenic mice (RID mice;...
Saved in:
Published in | Parasitology research (1987) Vol. 119; no. 6; pp. 1829 - 1843 |
---|---|
Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
24.03.2020
|
Online Access | Get full text |
Cover
Loading…
Summary: | The underlying pathogenic mechanisms of cardiomyopathy in Chagas Disease are still unsolved. In order to better clarify the role of fat on the evolution of cardiomyopathy, the present study employed three murine models of chronic
Trypanosoma cruzi
infection: (1) aP2-RIDα/β transgenic mice (RID mice; an adipose tissue model which express a gain-of function potent anti-inflammatory activity), (2) allograft inflammatory factor-1 knockout mice (Aif1
−/−
) and (3) a
Swiss
outbred mice. RID mice and non-transgenic mice (wild type – WT) were infected with blood trypomastigotes of Brazil strain. During acute stage RID showed lower parasitemia, lower heart inflammation and a decrease in the relative distribution of parasite load from cardiac muscle tissue towards epididymal fat. Nevertheless, comparable profiles of myocardial inflammatory infiltrates and relative distribution of parasite load were observed amongst RID and WT at chronic stage. Aif1
−/−
and Aif1
+/+
mice were infected with bloodstream trypomastigotes of Tulahuen strain and fed with high fat diet (HFD) or regular diet (RD). Interestingly, Aif1
+/+
HFD infected mice showed the highest mortality.
Swiss
mice infected with blood trypomastigotes of Berenice-78 strain on a HFD had higher levels of TNFα and more inflammation in their heart tissue than infected mice fed a RD. These various murine models implicate adipocytes in the pathogenesis of chronic Chagas Disease and suggest that HFD can lead to a significant increase in the severity of parasite-induced chronic cardiac damage. Furthermore, these data implicate adipocyte TLR4-, TNFα- and IL-1β- mediated signaling in pro-inflammatory pathways and Aif-1 gene expression in the development of chronic Chagas disease. |
---|---|
Bibliography: | Paul Zaki and Elisa LBC Domingues contributed equally to this article |
ISSN: | 0932-0113 1432-1955 |
DOI: | 10.1007/s00436-020-06645-z |