PROTEIN PHOSPHATASE 2A-B′γ Controls Botrytis cinerea Resistance and Developmental Leaf Senescence1[OPEN]

PP2A-B′γ interacts with CALCIUM-DEPENDENT PROTEIN KINASE1 and age-dependently controls salicylic acid-related signaling in plant immunity and developmental leaf senescence. Plants optimize their growth and survival through highly integrated regulatory networks that coordinate defensive measures and...

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Published inPlant physiology (Bethesda) Vol. 182; no. 2; pp. 1161 - 1181
Main Authors Durian, Guido, Jeschke, Verena, Rahikainen, Moona, Vuorinen, Katariina, Gollan, Peter J., Brosché, Mikael, Salojärvi, Jarkko, Glawischnig, Erich, Winter, Zsófia, Li, Shengchun, Noctor, Graham, Aro, Eva-Mari, Kangasjärvi, Jaakko, Overmyer, Kirk, Burow, Meike, Kangasjärvi, Saijaliisa
Format Journal Article
LanguageEnglish
Published American Society of Plant Biologists 28.10.2019
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Summary:PP2A-B′γ interacts with CALCIUM-DEPENDENT PROTEIN KINASE1 and age-dependently controls salicylic acid-related signaling in plant immunity and developmental leaf senescence. Plants optimize their growth and survival through highly integrated regulatory networks that coordinate defensive measures and developmental transitions in response to environmental cues. Protein phosphatase 2A (PP2A) is a key signaling component that controls stress reactions and growth at different stages of plant development, and the PP2A regulatory subunit PP2A‐B′γ is required for negative regulation of pathogenesis responses and for maintenance of cell homeostasis in short-day conditions. Here, we report molecular mechanisms by which PP2A‐B′γ regulates Botrytis cinerea resistance and leaf senescence in Arabidopsis ( Arabidopsis thaliana ). We extend the molecular functionality of PP2A‐B′γ to a protein kinase–phosphatase interaction with the defense-associated calcium-dependent protein kinase CPK1 and present indications this interaction may function to control CPK1 activity. In presenescent leaf tissues, PP2A-B′γ is also required to negatively control the expression of salicylic acid-related defense genes, which have recently proven vital in plant resistance to necrotrophic fungal pathogens. In addition, we find the premature leaf yellowing of pp2a - b′γ depends on salicylic acid biosynthesis via SALICYLIC ACID INDUCTION DEFICIENT2 and bears the hallmarks of developmental leaf senescence. We propose PP2A-B′γ age-dependently controls salicylic acid-related signaling in plant immunity and developmental leaf senescence.
Bibliography:www.plantphysiol.org/cgi/doi/10.1104/pp.19.00893
Present address: Microbial Biotechnology, Technical University of Munich Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, 94315 Straubing, Germany.
Present address: State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, 430062 Wuhan, China.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Saijaliisa Kangasjärvi (saijaliisa.kangasjarvi@utu.fi).
Senior author.
G.D., V.J., M.Bu., and S.K. designed the research; G.D., V.J., M.R., K.V., M.Br., and Z.W. performed the experiments; G.D., V.J., K.V., P.J.G., M.Br., J.S., and M.Bu. analyzed the data; G.D. developed the Arabidopsis-BiFC method; V.J. established a method for metabolite profiling; S.L. and G.N. provided the pp2a‐b′γ sid2 double mutant; J.K. provided the yeast two-hybrid library; G.D. and S.K. wrote the article with input from V.J., E.G., E.-M.A., J.K., K.O., and M.Bu.; S.K. agrees to serve as the author responsible for contact, and ensures communication.
Present address: Nanyang Technological University, Singapore 639798.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.19.00893