The Evening Complex Establishes Repressive Chromatin Domains Via H2A.Z Deposition1

The Evening Complex interacts with the complex responsible for the deposition of the histone variant H2A.Z, creating repressive chromatin domains to repress a cohort of target genes in Arabidopsis. The Evening Complex (EC) is a core component of the Arabidopsis ( Arabidopsis thaliana ) circadian clo...

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Published inPlant physiology (Bethesda) Vol. 182; no. 1; pp. 612 - 625
Main Authors Tong, Meixuezi, Lee, Kyounghee, Ezer, Daphne, Cortijo, Sandra, Jung, Jaehoon, Charoensawan, Varodom, Box, Mathew S., Jaeger, Katja E., Takahashi, Nozomu, Mas, Paloma, Wigge, Philip A., Seo, Pil Joon
Format Journal Article
LanguageEnglish
Published American Society of Plant Biologists 11.11.2019
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Summary:The Evening Complex interacts with the complex responsible for the deposition of the histone variant H2A.Z, creating repressive chromatin domains to repress a cohort of target genes in Arabidopsis. The Evening Complex (EC) is a core component of the Arabidopsis ( Arabidopsis thaliana ) circadian clock, which represses target gene expression at the end of the day and integrates temperature information to coordinate environmental and endogenous signals. Here we show that the EC induces repressive chromatin structure to regulate the evening transcriptome. The EC component ELF3 directly interacts with a protein from the SWI2/SNF2-RELATED (SWR1) complex to control deposition of H2A.Z-nucleosomes at the EC target genes. SWR1 components display circadian oscillation in gene expression with a peak at dusk. In turn, SWR1 is required for the circadian clockwork, as defects in SWR1 activity alter morning-expressed genes. The EC-SWR1 complex binds to the loci of the core clock genes PSEUDO - RESPONSE REGULATOR7 ( PRR7 ) and PRR9 and catalyzes deposition of nucleosomes containing the histone variant H2A.Z coincident with the repression of these genes at dusk. This provides a mechanism by which the circadian clock temporally establishes repressive chromatin domains to shape oscillatory gene expression around dusk.
Bibliography:www.plantphysiol.org/cgi/doi/10.1104/pp.19.00881
These authors contributed equally to this work.
Senior authors.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Pil Joon Seo (pjseo1@snu.ac.kr).
P.A.W., P.M., and P.J.S. participated in the design of the study and wrote the article; M.T., K.L., and N.T. performed the molecular experiments; M.T. and D.E. performed the analysis of sequencing data; M.T., S.C., J.J., V.C., and M.S.B. performed large-scale time-course experiments; M.T. and K.E.J. performed ChIP-seq experiments; P.A.W., P.M., and P.J.S. conceived the project; all authors read and approved the final article.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.19.00881