Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms1
The transcript levels of heavy-chain zein genes ( zH1 and zH2 ) and the occurrence of the zH polypeptides in different opaque-2 ( o2 ) lines were investigated by RNA-blot analyses and by sodium dodecylsulfate-polyacrylamide gel electrophoresis or two-dimensional gel electrophoresis protein fractiona...
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Published in | Plant physiology (Bethesda) Vol. 124; no. 1; pp. 451 - 460 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society of Plant Physiologists
01.09.2000
|
Online Access | Get full text |
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Summary: | The transcript levels of heavy-chain zein genes (
zH1
and
zH2
) and the occurrence of the zH polypeptides in different
opaque-2
(
o2
) lines were investigated by RNA-blot analyses and by sodium dodecylsulfate-polyacrylamide gel electrophoresis or two-dimensional gel electrophoresis protein fractionations. Four mutant alleles
o2R, o2T, o2It
, and
o2-676
introgressed into different genetic backgrounds (
GB
s) were considered. The mono-dimensional gel electrophoresis zein pattern can be either conserved or different among the various
GB
s carrying the same
o2
allele. Likewise, in the identical
GB
carrying different
o2
alleles, the zein pattern can be either conserved or differentially affected by the different mutant allele. Zein protein analysis of reciprocal crosses between lines with different
o2
alleles or the same
o2
showed in some case a more than additive zH pattern in respect to the
o2
parent lines. Electrophoretic mobility shift assay approaches, with O2-binding oligonucleotide and endosperm extracts from the above
o2
lines, failed to reveal o2-specific retarded band in any of the
o2
extracts. The results suggest that the promoter of some
zH1
and
zH2
contains motif(s) that can respond to factors other than O2. |
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Bibliography: | Corresponding author; e-mail aviotti@icm.mi.cnr.it; fax 39–2– 23699411. Present address: Department of Biology, University of California at San Diego, La Jolla, CA 92093–0116. |
ISSN: | 0032-0889 1532-2548 |