Polyketide bio-derivatization using the promiscuous acyltransferase KirCII

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses non-malon...

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Published inACS synthetic biology Vol. 6; no. 3; pp. 421 - 427
Main Authors Musiol-Kroll, Ewa M., Zubeil, Florian, Schafhauser, Thomas, Härtner, Thomas, Kulik, Andreas, McArthur, John, Koryakina, Irina, Wohlleben, Wolfgang, Grond, Stephanie, Williams, Gavin J., Lee, Sang Yup, Weber, Tilmann
Format Journal Article
LanguageEnglish
Published 22.02.2017
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Summary:During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses non-malonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo , the latter were utilized as educts for further modification by ‘click’ chemistry.
Bibliography:Present address: Department of Chemistry, University of California, One Shields Avenue, Davis, USA
Equal contribution
Present address: Intrexon Corporation, South San Francisco, California, USA
ISSN:2161-5063
DOI:10.1021/acssynbio.6b00341