Nutritional Homeostasis in Batch and Steady-State Culture of YeastD
We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae . We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures gr...
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Published in | Molecular biology of the cell Vol. 15; no. 9; pp. 4089 - 4104 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
The American Society for Cell Biology
01.09.2004
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Online Access | Get full text |
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Summary: | We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of
S. cerevisiae
. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same medium just before the limiting nutrient is completely exhausted. The massive stress response and complete arrest of the cell cycle that occurs when nutrients are fully exhausted in batch cultures is not observed in the chemostat, indicating that the cells in the chemostat are “poor, not starving.” Similar comparisons using leucine or uracil auxotrophs limited on leucine or uracil again showed patterns of gene expression in steady-state closely resembling those of corresponding batch cultures just before they exhaust the nutrient. Although there is also a strong stress response in the auxotrophic batch cultures, cell cycle arrest, if it occurs at all, is much less uniform. Many of the differences among the patterns of gene expression between the four nutrient limitations are interpretable in light of known involvement of the genes in stress responses or in the regulation or execution of particular metabolic pathways appropriate to the limiting nutrient. We conclude that cells adjust their growth rate to nutrient availability and maintain homeostasis in the same way in batch and steady state conditions; cells in steady-state cultures are in a physiological condition normally encountered in batch cultures. |
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Bibliography: | Present address: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544. Corresponding author. E-mail address: botstein@princeton.edu. Online version of this article contains supporting material. Online version is available at www.molbiolcell.org. Present address: Division of Biology, California Institute of Technology, Pasadena, CA 81125 Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E04-04-0306. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-04-0306. |
ISSN: | 1059-1524 |
DOI: | 10.1091/mbc.E04-04-0306 |