Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi † †Electronic supplementary information (ESI) available: Figures and tables. See DOI: 10.1039/c5sc03340c Click here for additional data file
Bio-orthogonal non-canonical amino acid tagging allows time-resolved proteomic analysis of quorum sensing in Vibrio harveyi . Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell...
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Published in | Chemical science (Cambridge) Vol. 7; no. 3; pp. 1797 - 1806 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Royal Society of Chemistry
23.11.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Bio-orthogonal non-canonical amino acid tagging allows time-resolved proteomic analysis of quorum sensing in
Vibrio harveyi
.
Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism
Vibrio harveyi
by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of
V. harveyi
from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis. |
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ISSN: | 2041-6520 2041-6539 |
DOI: | 10.1039/c5sc03340c |