Transcription Factor ATAF1 in Arabidopsis Promotes Senescence by Direct Regulation of Key Chloroplast Maintenance and Senescence Transcriptional Cascades1[OPEN]
A transcription factor from Arabidopsis antagonistically controls regulators of senescence and photosynthesis. Senescence represents a fundamental process of late leaf development. Transcription factors ( TF s) play an important role for expression reprogramming during senescence; however, the gene...
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| Published in | Plant physiology (Bethesda) Vol. 168; no. 3; pp. 1122 - 1139 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
American Society of Plant Biologists
07.05.2015
|
| Online Access | Get full text |
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| Summary: | A transcription factor from Arabidopsis antagonistically controls regulators of senescence and photosynthesis.
Senescence represents a fundamental process of late leaf development. Transcription factors (
TF
s) play an important role for expression reprogramming during senescence; however, the gene regulatory networks through which they exert their functions, and their physiological integration, are still largely unknown. Here, we identify the Arabidopsis (
Arabidopsis thaliana
) abscisic acid (
ABA
)- and hydrogen peroxide-activated
TF
Arabidopsis thaliana ACTIVATING FACTOR1
(
ATAF1
) as a novel upstream regulator of senescence. ATAF1 executes its physiological role by affecting both key chloroplast maintenance and senescence-promoting
TF
s, namely
GOLDEN2-LIKE1
(
GLK1
) and
ORESARA1
(
ARABIDOPSIS NAC092
), respectively. Notably, while ATAF1 activates
ORESARA1
, it represses
GLK1
expression by directly binding to their promoters, thereby generating a transcriptional output that shifts the physiological balance toward the progression of senescence. We furthermore demonstrate a key role of ATAF1 for
ABA
- and hydrogen peroxide-induced senescence, in accordance with a direct regulatory effect on
ABA
homeostasis genes, including
NINE-CIS-EPOXYCAROTENOID DIOXYGENASE3
involved in ABA biosynthesis and
ABC TRANSPORTER G FAMILY MEMBER40
, encoding an ABA transport protein. Thus, ATAF1 serves as a core transcriptional activator of senescence by coupling stress-related signaling with photosynthesis- and senescence-related transcriptional cascades. |
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| Bibliography: | S.B. designed the research and supervised the group; S.M.-B. performed the hormone analysis; G.-P.X. performed the CELD experiment; P.G. and S.B. performed all other experiments; S.B. wrote the article with contributions from the other authors. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Salma Balazadeh (balazadeh@mpimp-golm.mpg.de). www.plantphysiol.org/cgi/doi/10.1104/pp.15.00567 |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.15.00567 |