The Arabidopsis ABHD11 Mutant Accumulates Polar Lipids in Leaves as a Consequence of Absent Acylhydrolase Activity1[OPEN]
Disruption of the ABHD11 hydrolase causes polar lipids accumulation, thereby enhancing plant growth. Alpha/beta hydrolase domain (ABHD)-containing proteins are structurally related with diverse catalytic activities. In various species, some ABHD proteins have been characterized and shown to play rol...
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Published in | Plant physiology (Bethesda) Vol. 170; no. 1; pp. 180 - 193 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
American Society of Plant Biologists
20.11.2015
|
Online Access | Get full text |
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Summary: | Disruption of the ABHD11 hydrolase causes polar lipids accumulation, thereby enhancing plant growth.
Alpha/beta hydrolase domain (ABHD)-containing proteins are structurally related with diverse catalytic activities. In various species, some ABHD proteins have been characterized and shown to play roles in lipid homeostasis. However, little is known about ABHD proteins in plants. Here, we characterized AT4G10030 (
AtABHD11
), an Arabidopsis (
Arabidopsis thaliana
) homolog of a human
ABHD11
gene. In silico analyses of AtABHD11 revealed homology with other plant species with a conserved G
X
S
X
G lipid motif. Interestingly, Arabidopsis
abhd11
mutant plants exhibited an enhanced growth rate compared with wild-type plants. Quantitative analyses of the total lipids showed that the mutant
abhd11
has a high amount of phospholipid and galactolipid in Arabidopsis leaves. The overexpression of
AtABHD11
in
Escherichia coli
led to a reduction in phospholipid levels. The bacterially expressed recombinant AtABHD11 hydrolyzed lyso(phospho)lipid and monoacylglycerol. Furthermore, using whole-genome microarray and real-time PCR analyses of
abhd11
and wild-type plants, we noted the up-regulation of
MGD1
,
-2
, and -
3
and
DGD1
. Together, these findings suggested that AtABHD11 is a lyso(phospho)lipase. The disruption of
AtABHD11
caused the accumulation of the polar lipids in leaves, which in turn promoted a higher growth rate compared with wild-type plants. |
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Bibliography: | The author is responsible for the distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Ram Rajasekharan (ram@cftri.com). www.plantphysiol.org/cgi/doi/10.1104/pp.15.01615 |
ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.15.01615 |