Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays ǂ

We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-anti...

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Published inBiosensors (Basel) Vol. 5; no. 2; pp. 158 - 171
Main Authors Miller, John, Castaneda, Angelica, Lee, Kun Ho, Sanchez, Martin, Ortiz, Adrian, Almaz, Ekrem, Turkoglu Almaz, Zuleyha, Murinda, Shelton, Lin, Wei-Jen, Salik, Ertan
Format Journal Article
LanguageEnglish
Published MDPI 01.04.2015
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Summary:We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G)-antigen (rabbit anti-mouse IgG) reactions was studied. Hydrofluoric acid treatment makes the sensitive region thinner to enhance sensitivity, which we confirmed by experiments and simulations. The limit of detection for the sensor was estimated to be less than 50 ng/mL. Utilization of the rate of the sensor peak shift within the first few minutes of the antibody-antigen reaction is proposed as a rapid protein detection method.
Bibliography:These authors contributed equally to this work.
Based on “Miller, J.; Castaneda, A.; Lee, K.H.; Sanchez, M.; Murinda, S.; Lin, W.-J.; Salik, E. Biconically Tapered Fiber Optic Dip Probe for Rapid Label—Free Immunoassays. In Proceedings of Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV Israel Gannot, San Francisco, CA, USA, 1 February 2014”.
ISSN:2079-6374
DOI:10.3390/bios5020158