Microsatellite primers for the fungi Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) from 454 shotgun pyrosequencing1
• Premise of the study: Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) are ectomycorrhizal fungi symbiotic with pines and the mycoheterotrophic plant Pterospora andromedea (Ericaceae). Microsatellite loci will allow population genetic study of fungal hosts to P. andromedea . • Methods and...
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Published in | Applications in plant sciences Vol. 2; no. 7 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Botanical Society of America
10.07.2014
|
Subjects | |
Online Access | Get full text |
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Summary: | •
Premise of the study: Rhizopogon kretzerae
and
R. salebrosus
(Rhizopogonaceae) are ectomycorrhizal fungi symbiotic with pines and the mycoheterotrophic plant
Pterospora andromedea
(Ericaceae). Microsatellite loci will allow population genetic study of fungal hosts to
P. andromedea
.
•
Methods and Results:
Shotgun pyrosequencing of
R. kretzerae
DNA resulted in primer development of 23 perfect microsatellite loci and screened across two populations each for
R. kretzerae
and
R. salebrosus
. Twelve loci were polymorphic in
R. kretzerae
populations, and 11 loci cross-amplified in
R. salebrosus
populations. For
R. kretzerae
and
R. salebrosus
, number of alleles was one to eight and one to nine, respectively, and observed heterozygosity ranged from 0.00–0.57 and 0.00–0.70, respectively.
•
Conclusions:
These are the first microsatellite loci developed for any species within
Rhizopogon
subgenus
Amylopogon
. These microsatellite loci will be used in conservation genetic studies of rare to endangered eastern populations and to compare plant and fungal population genetic structure at different hierarchical levels. |
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Bibliography: | Funding for this research has been provided by the National Science Foundation (DEB-1050315 to M.R.K. and DEB-1050292 to S.L.M.). |
ISSN: | 2168-0450 |
DOI: | 10.3732/apps.1400029 |