Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Affinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement1

CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities...

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Published inThe Journal of immunology (1950) Vol. 171; no. 9; pp. 4493 - 4503
Main Authors Kerry, Samantha E., Buslepp, Jennifer, Cramer, Lorraine A., Maile, Robert, Hensley, Lucinda L., Nielsen, Alma I., Kavathas, Paula, Vilen, Barbara J., Collins, Edward J., Frelinger, Jeffrey A.
Format Journal Article
LanguageEnglish
Published 01.11.2003
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Summary:CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal over time allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.
ISSN:0022-1767
1550-6606