Data mining cDNAs reveals 3 new ssRNA viruses in Nasonia (Hymenoptera: Pteromalidae)

We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia . The genome of this kind of virus is a positive-sense single-stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-...

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Published inInsect molecular biology Vol. 19; no. Suppl 1; pp. 99 - 107
Main Authors Oliveira, Deodoro C.S.G., Hunter, Wayne B., Ng, Julienne, Desjardins, Christopher A., Dang, Phat M., Werren, John H.
Format Journal Article
LanguageEnglish
Published 01.02.2010
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Summary:We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia . The genome of this kind of virus is a positive-sense single-stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales . A third virus, NvitV-3, is most similar to the Nora virus from Drosophila . A RT-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia .
Bibliography:present address: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, BCN 08193, Spain.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2009.00934.x