Three-dimensional Structure of A1A0 ATP Synthase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Electron MicroscopyS
The archaeal ATP synthase is a multisubunit complex that consists of a catalytic A 1 part and a transmembrane, ion translocation domain A 0 . The A 1 A 0 complex from the hyperthermophile Pyrococcus furiosus was isolated. Mass analysis of the complex by laser-induced liquid bead ion desorption (LILB...
Saved in:
Published in | The Journal of biological chemistry Vol. 284; no. 15; pp. 10110 - 10119 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
10.04.2009
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The archaeal ATP synthase is a multisubunit complex that consists of a
catalytic A
1
part and a transmembrane, ion translocation domain
A
0
. The A
1
A
0
complex from the
hyperthermophile
Pyrococcus furiosus
was isolated. Mass analysis of
the complex by laser-induced liquid bead ion desorption (LILBID) indicated a
size of 730 ± 10 kDa. A three-dimensional map was generated by electron
microscopy from negatively stained images. The map at a resolution of 2.3 nm
shows the A
1
and A
0
domain, connected by a central stalk
and two peripheral stalks, one of which is connected to A
0
, and
both connected to A
1
via prominent knobs. X-ray structures of
subunits from related proteins were fitted to the map. On the basis of the
fitting and the LILBID analysis, a structural model is presented with the
stoichiometry
A
3
B
3
CDE
2
FH
2
ac
10
. |
---|---|
Bibliography: | To whom correspondence should be addressed: Max-Planck-Institute of Biophysics, Max-von-Laue-Str. 3, D-60438 Frankfurt am Main, Germany. E-mail: janet.vonck@mpibp-frankfurt.mpg.de. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 472, to V. M. and Werner Kühlbrandt). The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. S1. The three-dimensional EM map has been deposited in the EM Data Base (http://www.ebi.ac.uk/mds/index.html) with the accession number EMD-1542. |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M808498200 |