Sequencing along DNA fragments cloned in bacteriophage M13 by using internal primers. The sequence analysis of a yeast DNA fragment containing a replication origin

In the `shotgun' procedure for sequencing DNA, DNA fragments are cloned into a phage M13 vector and sequenced by using a flanking primer. In a variation of this procedure a longer DNA sequence is cloned into M13, the two single-stranded recombinants identified and sequenced by using a set of in...

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Bibliographic Details
Published inBiochemical journal Vol. 199; no. 3; pp. 819 - 823
Main Authors Hindley, John, Phear, Geraldine A.
Format Journal Article
LanguageEnglish
Published 01.12.1981
Subjects
Gene Structure and Function
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Summary:In the `shotgun' procedure for sequencing DNA, DNA fragments are cloned into a phage M13 vector and sequenced by using a flanking primer. In a variation of this procedure a longer DNA sequence is cloned into M13, the two single-stranded recombinants identified and sequenced by using a set of internal primers prepared by exonuclease III digestion of restriction fragments.
ISSN:0264-6021
1470-8728