Identification of Endogenous Gibberellins from Sorghum1

Gibberellins (GA) A 1 , A 19 , and A 20 were identified in shoot cylinders containing the apical meristems from sorghum ( Sorghum bicolor L.). Extracts were purified by sequential SiO 2 partition chromatography and reversed-phase C 18 high performance liquid chromatography and biologically active (d...

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Published inPlant physiology (Bethesda) Vol. 82; no. 1; pp. 330 - 332
Main Authors Rood, Stewart B., Larsen, Karen M., Mander, Lewis N., Abe, Hiroshi, Pharis, Richard P.
Format Journal Article
LanguageEnglish
Published 01.09.1986
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Summary:Gibberellins (GA) A 1 , A 19 , and A 20 were identified in shoot cylinders containing the apical meristems from sorghum ( Sorghum bicolor L.). Extracts were purified by sequential SiO 2 partition chromatography and reversed-phase C 18 high performance liquid chromatography and biologically active (dwarf rice cv Tan-ginbozu microdrop assay) fractions were subjected to gas chromatography-selected ion monitoring. Based on the use of [ 3 H]GA and [ 2 H](d 2 )GA internal standards, amounts of GA 1 , GA 19 , and GA 20 were estimated to be 0.7, 8.8, and 1.5 namograms per gram dry weight of tissue, respectively.
Bibliography:Supported through Natural Sciences and Engineering Research Council grants U0286 and A-2585 to SBR and RPP, respectively.
ISSN:0032-0889
1532-2548