Temperature-Sensitive Alleles of RSW2 Link the KORRIGAN Endo-1,4-β-Glucanase to Cellulose Synthesis and Cytokinesis in Arabidopsis1
An 8.5-kb cosmid containing the KORRIGAN gene complements the cellulose-deficient rsw2-1 mutant of Arabidopsis. Three temperature-sensitive alleles of rsw2 show single amino acid mutations in the putative endo-1,4-β-glucanase encoded by KOR . The F 1 from crosses between kor-1 and rsw2 alleles shows...
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Published in | Plant physiology (Bethesda) Vol. 126; no. 1; pp. 278 - 288 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society of Plant Physiologists
01.05.2001
|
Online Access | Get full text |
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Summary: | An 8.5-kb cosmid containing the
KORRIGAN
gene complements the cellulose-deficient
rsw2-1
mutant of Arabidopsis. Three temperature-sensitive alleles of
rsw2
show single amino acid mutations in the putative endo-1,4-β-glucanase encoded by
KOR
. The F
1
from crosses between
kor-1
and
rsw2
alleles shows a weak, temperature-sensitive root phenotype. The shoots of
rsw2-1
seedlings produce less cellulose and accumulate a short chain, readily extractable glucan resembling that reported for
rsw1
(which is defective in a putative glycosyltransferase required for cellulose synthesis). The double mutant (
rsw2-1 rsw1
) shows further reductions in cellulose production relative to both single mutants, constitutively slow root growth, and enhanced temperature-sensitive responses that are typically more severe than in either single mutant. Abnormal cytokinesis and severely reduced birefringent retardation in elongating root cell walls of
rsw2
link the enzyme to cellulose production for primary cell walls and probably cell plates. The Rsw2
−
phenotype generally resembles the Kor
−
and cellulose-deficient Rsw1
−
phenotypes, but anther dehiscence is impaired in Rsw2-1
−
. The findings link a second putative enzyme activity to cellulose synthesis in primary cell walls of Arabidopsis and further increases the parallels to cellulose synthesis in
Agrobacterium tumefaciens
where the
celA
and
celC
genes are required and encode a putative glycosyltransferase and an endo-1,4-β-glucanase related to RSW1 and KOR, respectively. |
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Bibliography: | Corresponding author: e-mail richard@rsbs.anu.edu.au; fax 61–2–6249–4331. Present address: Cellular and Structural Biology, University of Colorado Health Sciences Center, B–111 4200 E. 9th Avenue, Denver, CO 80262. Present address: Aventis Pty Ltd, G.P.O. Box 1600, Canberra, ACT 2601, Australia Present address: The Plant Gene Expression Center, U.S. Department of Agriculture/Agricultural Research Service, University of California/Berkeley, 800 Buchanan Street, Albany, CA 94710. |
ISSN: | 0032-0889 1532-2548 |