Characteristics of Listeria monocytogenes strains isolated in Russia and their typing using pulse electrophoresis

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 3; p. 60
• Main Authors: Marakusha, B I, Darwich, K, Tartakovskiĭ, I S
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.1996
• Subjects: Animals; Bacterial Typing Techniques - statistics & numerical data; Chromosomes, Bacterial - genetics; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field - methods; Electrophoresis, Gel, Pulsed-Field - statistics & numerical data; Food Microbiology; Guinea Pigs; Humans; Listeria monocytogenes - classification; Listeria monocytogenes - genetics; Listeria monocytogenes - isolation & purification; Listeria monocytogenes - pathogenicity; Mice; Restriction Mapping; Russia; Sewage - microbiology; Virulence; Russia
• ISSN: 0372-9311; 2686-7613

On the basis of specially developed scheme for the isolation of Listeria strains comprising 2 enrichment stages and the use of growth inhibitors, 128 L. monocytogenes cultures were isolated from clinical material, foodstuffs and sewage water. Highly virulent L.monocytogenes strains isolated from clinical material belonged to serovar 4b (54%) and 1/2a (38%), while those isolated from foodstuffs and sewage water belonged to 4b (74%). The restriction analysis of the chromosomal DNA of the isolated cultures with the use of restrictase EcoR1 on the basis of pulsed-field gel electrophoresis (PFGE) made it possible to distinguish Listeria strains in accordance with 5 types of restrictograms. The restrictograms of highly virulent L. monocytogenes strains, serovar 4b, belonged to types 1 and 2, while those of L. monocytogenes strains, serovar 1/2a, belonged to types 2 and 3. The comparative use of different methods for typing L. monocytogenes (sero-, phago-, bio- and resistotyping, the analysis of plasmid composition and restriction analysis) revealed that the combination of serotyping and restriction analysis on the basis of PFGE proved to be most promising for the characterization of the isolated L. monocytogenes strains and the assessment of their epidemic importance.